November 18, 2011

Novel C1q assay reveals a clinically relevant subset of HLA antibodies independent of immunoglobulin G strength on single antigen beads


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The first pre-transplant cross-matches were performed using complement-dependent cytotoxicity (CDC) and showed immediate clinical benefits. Current HLA antibody test methods rely on Luminex single antigen bead (SAB) technology allowing for the sensitive identification of multiple DSAs even in highly sensitized patients. However, the clinical relevance of the HLA antibodies identified by the Luminex platform remains ill defined. The addition of C1q detection to the Luminex assay allows for the identification of complement-fixing HLA antibodies. This manuscript describes details of this assay and compares data to normal Luminex results and CDC results. It also shows data from a small number of clinical cases where antibodies detected by C1q better correlated with clinical outcomes than antibodies normally detected by Luminex.

Cardiac Transplantation, Liver Transplantation, Lung Transplantation, Renal Transplantation

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